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Image Search Results
Journal: EMBO Reports
Article Title: Dynamic SAS-6 phosphorylation aids centrosome duplication and elimination in C. elegans oogenesis
doi: 10.1038/s44319-025-00485-7
Figure Lengend Snippet: ( A ) MPM-2 Immunostaining in the germline of worms treated with DMSO or PA. DNA was stained with DAPI (blue). Scale bar, 20 µm. ( B ) Quantification of relative MPM-2 intensity in the germline of worms treated with DMSO or PA. Each point represents the average relative MPM-2 intensity of cells at a specific stage within a gonad. Data are shown as mean ± SEM. P values were determined using two-tailed unpaired t-tests. At least seven gonads were measured. ( C ) GFP::SAS-5 focus maintenance in the germline of worms treated with DMSO or CDK-1 inhibitors. DNA was stained with DAPI (blue). Insects are 2-fold magnified images of the detected foci, and undetected foci are marked with cross symbols. The negative numbers indicate oocyte position as depicted in Fig. . Scale bar, 10 µm. ( D ) Quantification of GFP::SAS-5 focus fluorescence intensity and maintenance in oocytes of worms treated as in ( C ). The numbers of gonad arms scored are: DMSO, n = 10; PA, n = 10; RO3306, n = 13. ( E ) GFP::SAS-7 focus maintenance in the germline of worms treated with DMSO or CDK-1 inhibitors. DNA was stained with DAPI (blue). Scale bar, 10 µm. ( F ) Quantification of GFP::SAS-7 focus fluorescence intensity and maintenance in oocytes of worms treated as in ( E ). The numbers of gonad arms scored are: DMSO, n = 15; PA, n = 13; RO3306, n = 14. .
Article Snippet: To identify the phosphorylation sites of SAS-6 in vivo, synchronized young adult worms were shredded by vortexing in 15-ml conical tube containing small sharp broken glass coverslips in ice-cold lysis buffer (150 mM NaCl, 20 mM KCl, 1.5 mM MgCl 2 , 20 mM Tris-HCl pH 7.5, 1% NP-40) with
Techniques: Immunostaining, Staining, Two Tailed Test, Fluorescence